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) image analysis, 194202 multichannel segmentation techniques, 2023 multiplexing, 19294 subcellular, 2034 summary, 204 Quantitative analysis, 1067 data preprocessing, 106 model fitting, 1067 motion assessment and tracking, 107 pattern recognition and classification, 107 Quantitative phenotyping, 35784 biomedical applications, 35961 introduction, 35759 with microscopic images, 35784 mouse mammary glad phenotyping study, 38284 mouse model study, 35960 mouse placenta phenotyping studies, 38082 segmentation of individual cells, 36474 segmentation with N-point correlation functions, 36164 summary, 384 3-D visualization, 37580 validation, 38084 workflow, 358 zebrafish embryo, 36061 Quantization precision, 95 Quantum dots, 20, 3436 anatomy, 35 in confocal microscopy, 36 defined, 34, 107 illumination, 34 multiplexing, 19394 photostability, 36 spectral profiles, 35 in widefield fluorescence microscopy, 36 Quantum efficiency, 95 Quenching collisional agents, 41 common example, 41 consequences, 40 defined, 4041 static (complex), 41 R Rab-GTPases, 170, 171 Radial basis function (RBF), 289 Random walk (RW), 259 Real intermediate image plane (RIIP), 5 Real-time fiber pattern rejection, 44750 calibrated raw data acquisition, 44748 calibration, 449 imaging model, 449 real-time processing, 44850 reconstruction, 450 Real-time focusing algorithm, 106 ReAsH, 40 Receiver operating characteristic (ROC) curves, 416, 418 Recombinant proteins, 1078 Red blood cells (RBCs), 45153 Reflected light microscopy, 1012 contrast mechanisms, 1112 defined, 10 forex robot plus500 diagram, 10 See also Microscopy Region adjacency graph, 126 Region-based segmentation, 200 Regions of interest (ROI), 285 rectangular, 457 tracking, 454, 456 Region tracking, 45457 affine registration algorithm, 455 application to cell trafficking, 45657 motion compensation algorithm, 45455 Registration, 7273 disparity function evaluation, 431 dynamic model for, 437 elastic, 106 image, 19799 importance, 371 of large microscopic images, 37174 of MicroCT images, 42938 neuron morphology reconstruction, 394 nonrigid, 37274 observation model for, 438 184 E.

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